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Factor X (cleaved Ile235) antibody

Anti-Factor X (cleaved Ile235) antibody used in Western Blot (WB). GTX86907

Cat No. GTX86907

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Rat
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500~1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Product Note

Factor X (cleaved Ile235) antibody detects endogenous levels of fragment of activated FA10 resulting from cleavage adjacent to Ile235.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Factor X (216-265).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

F10 , FX , FXA , coagulation factor X , Factor X

Cellular Localization

Secreted

Background

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Factor X (cleaved Ile235) antibody used in Western Blot (WB). GTX86907

GTX86907 WB Image

WB analysis of rat liver cell lysates using GTX86907 Factor X (cleaved Ile235) antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

There are currently no references for Factor X (cleaved Ile235) antibody (GTX86907). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379