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Factor XII Light chain antibody [C6B7]

Orthogonal
Anti-Factor XII Light chain antibody [C6B7] used in Western Blot (WB). GTX21008

Cat. No. GTX21008

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C6B7

Isotype

 IgG1

Application

 Coagulation assay, WB, ELISA

Reactivity

 Human, Mouse
Package
125 μg ($399)
See all Factor XII products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Coagulation assay Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human Factor XII.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_374291

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F12 , HAE3 , HAEX , HAF , coagulation factor XII , FA12 , Factor XII

Cellular Localization

Secreted

Background

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Factor XII Light chain antibody [C6B7] used in Western Blot (WB). GTX21008

GTX21008 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 27248165

REFERENCE

Application Reference

Silasi R et al. Blood 2021; Factor XII plays a pathogenic role in organ failure and death in baboons challenged with Staphylococcus aureus.
Yang L et al. Oncotarget 2016; 7 (27) : 40919-40938 A plasma proteolysis pathway comprising blood coagulation proteases.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Package List Price ($)
$ 399