PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Colon: A fraction of monocytic cells in the mucosa should show a strong cytoplasmic factor XIIIa immunostaining.
Negative Control
Colon: Factor XIIIa immunostaining should be absent in epithelial, endothelial and smooth muscle cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Factor XIIIa protein (aa46-181)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
coagulation factor XIII A chain , F13A
Cellular Localization
Cytoplasm , Secreted
Background
This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04452 IHC-P Image
IHC-P analysis of human Hodgkin's lymphoma tissue using GTX04452 Factor XIIIa antibody [MSVA-813R] HistoMAX™. Hodgkin‘s lymphoma containing a dense population of factor XIIIa positive cells.
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GTX04452 IHC-P Image
IHC-P analysis of human mucosa from appendix tissue using GTX04452 Factor XIIIa antibody [MSVA-813R] HistoMAX™. A fraction of monocytic cells in appendix mucosa shows a strong cytoplasmic factor XIIIa immunostaining.
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GTX04452 IHC-P Image
IHC-P analysis of human lymph node tissue using GTX04452 Factor XIIIa antibody [MSVA-813R] HistoMAX™. Strong factor XIII immunostaining of sinus histiocytes in a lymph node.
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REFERENCE
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REVIEW
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