APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/25-1/50 |
| Assay dependent |
| Assay dependent |
|
Note :
FACS
The results may be optimized using membrane permeabilisation. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
Product Note
Clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fas ligand , ALPS1B , APT1LG1 , APTL , CD178 , CD95-L , CD95L , FASL , TNFSF6 , TNLG1A
Cellular Localization
Cell membrane
Background
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
![Anti-Fas Ligand antibody [14C2] used in ELISA (ELISA). GTX42343](/templates/default/js/lightbox/images/loading.gif "Anti-Fas Ligand antibody [14C2] used in ELISA (ELISA). GTX42343")
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GTX42343 ELISA Image
Sandwich ELISA analysis of human Fas Ligand binding using Mouse anti Human Fas Ligand (GTX42343) as a capture reagent and Mouse anti Human Fas Ligand (GTX42345) as a detection reagent with purified human Fas Ligand as antigen for the generation of a standard curve. Plasma (orange) sample is displayed at 1:2 dilution.
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GTX42343 FACS Image
FACS analysis of Jurkat cells using GTX42343 Fas Ligand antibody [14C2].
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REFERENCE
There are currently no references for Fas Ligand antibody [14C2] (GTX42343). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fas Ligand antibody [14C2] (GTX42343). Be the first to share your experience with this product.
![Anti-Fas Ligand antibody [14C2] used in ELISA (ELISA). GTX42343](/upload/website/prouct_img/small/GTX42343/GTX42343_3674_ELISA_w_23051500_969.webp "Anti-Fas Ligand antibody [14C2] used in ELISA (ELISA). GTX42343")
![Anti-Fas Ligand antibody [14C2] used in Flow cytometry (FACS). GTX42343](/upload/website/prouct_img/small/GTX42343/GTX42343_3671_FACS_w_23051500_436.webp "Anti-Fas Ligand antibody [14C2] used in Flow cytometry (FACS). GTX42343")
![Anti-Fas Ligand antibody [14C2] used in ELISA (ELISA). GTX42343](/upload/website/prouct_img/normal/GTX42343/GTX42343_3674_ELISA_w_23051500_969.webp "Anti-Fas Ligand antibody [14C2] used in ELISA (ELISA). GTX42343")
![Anti-Fas Ligand antibody [14C2] used in Flow cytometry (FACS). GTX42343](/upload/website/prouct_img/normal/GTX42343/GTX42343_3671_FACS_w_23051500_436.webp "Anti-Fas Ligand antibody [14C2] used in Flow cytometry (FACS). GTX42343")