PRODUCT
Summary
The NOK-1 antibody reacts with human CD178 (Fas ligand) in both membrane bound and soluble forms. Fas ligand is a 40 kDa transmembrane glycoprotein, a member of the TNF-α family, and is expressed by activated T and NK cells, neutrophils, and monocytes. Interactions between CD178 (Fas ligand) and CD95 (Fas) induce a program of apoptosis and play a key role in immune regulation and homeostasis. The extracellular domain of human CD178 can be cleaved from the surface by matrix metalloproteinases (MMPs) resulting in a 26 kDa soluble protein.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.05 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fas ligand , ALPS1B , APT1LG1 , APTL , CD178 , CD95L , FASL , FASLG , TNFSF6 , TNLG1A , fasL , Fas Ligand , Fasligand , CD95 ligand
Cellular Localization
Cell membrane,Cytoplasmic vesicle lumen,Lysosome lumen,Secreted,Nucleus
Background
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
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GTX01476-07 FACS Image
FACS analysis of human CD178 (Fas ligand) transfected cells using GTX01476-07 Fas Ligand antibody [NOK-1] (APC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 μg (5 μl)
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REFERENCE
REVIEW
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