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Fas Ligand antibody [NOK-1] (PE)

Anti-Fas Ligand antibody [NOK-1] (PE) used in Flow cytometry (FACS). GTX01476-08

Cat. No. GTX01476-08

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NOK-1

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 test ($239)
See all Fas Ligand products
PRODUCT

Summary

The NOK-1 antibody reacts with human CD178 (Fas ligand) in both membrane bound and soluble forms. Fas ligand is a 40 kDa transmembrane glycoprotein, a member of the TNF-α family, and is expressed by activated T and NK cells, neutrophils, and monocytes. Interactions between CD178 (Fas ligand) and CD95 (Fas) induce a program of apoptosis and play a key role in immune regulation and homeostasis. The extracellular domain of human CD178 can be cleaved from the surface by matrix metalloproteinases (MMPs) resulting in a 26 kDa soluble protein.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fas ligand , ALPS1B , APT1LG1 , APTL , CD178 , CD95L , FASL , FASLG , TNFSF6 , TNLG1A , fasL , Fas Ligand , Fasligand , CD95 ligand

Cellular Localization

Cell membrane,Cytoplasmic vesicle lumen,Lysosome lumen,Secreted,Nucleus

Background

This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Fas Ligand antibody [NOK-1] (PE) used in Flow cytometry (FACS). GTX01476-08

GTX01476-08 FACS Image

FACS analysis of human CD178 (Fas ligand) transfected cells using GTX01476-08 Fas Ligand antibody [NOK-1] (PE).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.5 μg (5 μl)

REFERENCE

Clone Reference

2019; Fatty Acid Synthase Contributes to Restimulation-Induced Cell Death of Human CD4 T Cells.
1995; Metalloproteinase-mediated release of human Fas ligand.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 239