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Fas Ligand antibody [NOK-1]

Cat. No. GTX01476

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NOK-1

Isotype

 IgG1

Application

 WB, FACS, IP, ELISA, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($189)
See all Fas Ligand products
PRODUCT

Summary

The NOK-1 antibody reacts with human CD178 (Fas ligand) in both membrane bound and soluble forms. Fas ligand is a 40 kDa transmembrane glycoprotein, a member of the TNF-α family, and is expressed by activated T and NK cells, neutrophils, and monocytes. Interactions between CD178 (Fas ligand) and CD95 (Fas) induce a program of apoptosis and play a key role in immune regulation and homeostasis. The extracellular domain of human CD178 can be cleaved from the surface by matrix metalloproteinases (MMPs) resulting in a 26 kDa soluble protein.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fas ligand , ALPS1B , APT1LG1 , APTL , CD178 , CD95L , FASL , FASLG , TNFSF6 , TNLG1A , fasL , Fas Ligand , Fasligand , CD95 ligand

Cellular Localization

Cell membrane,Cytoplasmic vesicle lumen,Lysosome lumen,Secreted,Nucleus

Background

This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]

Database

Research Area

REFERENCE

Clone Reference

2019; Fatty Acid Synthase Contributes to Restimulation-Induced Cell Death of Human CD4 T Cells.
Application : WB
Reactivity : Human
N Kayagaki et al. et al. J Exp Med. 1995; 182 (6):1777–1783 Metalloproteinase-mediated release of human Fas ligand
Application : FACS, IP, ELISA, Neutralizing/Inhibition
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 189