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Fas antibody [4F8H6]

Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557
Anti-Fas antibody [4F8H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60557
Anti-Fas antibody [4F8H6] used in Flow cytometry (FACS). GTX60557
Anti-Fas antibody [4F8H6] used in ELISA (ELISA). GTX60557
Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557

Cat No. GTX60557

Host

Mouse

Clonality

Monoclonal

Clone Name

4F8H6

Isotype

IgG1

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human FAS expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fas cell surface death receptor , ALPS1A , APO-1 , APT1 , CD95 , FAS1 , FASTM , TNFRSF6

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557

GTX60557 WB Image

WB analysis of human FAS (AA: 87-278) recombinant protein using GTX60557 Fas antibody [4F8H6].

Anti-Fas antibody [4F8H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60557

GTX60557 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60557 Fas antibody [4F8H6].
Green : Fas
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Fas antibody [4F8H6] used in Flow cytometry (FACS). GTX60557

GTX60557 FACS Image

FACS analysis of HeLa cells using GTX60557 Fas antibody [4F8H6].
Green : Fas
Red : negative control

Anti-Fas antibody [4F8H6] used in ELISA (ELISA). GTX60557

GTX60557 ELISA Image

ELISA analysis of antigen using GTX60557 Fas antibody [4F8H6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557

GTX60557 WB Image

WB analysis of HeLa (1), Jurkat (2) cell lysate using GTX60557 Fas antibody [4F8H6].

REFERENCE

There are currently no references for Fas antibody [4F8H6] (GTX60557). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339