APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human FAS expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fas cell surface death receptor , ALPS1A , APO-1 , APT1 , CD95 , FAS1 , FASTM , TNFRSF6
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
![Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557](/templates/default/js/lightbox/images/loading.gif "Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557")
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GTX60557 WB Image
WB analysis of human FAS (AA: 87-278) recombinant protein using GTX60557 Fas antibody [4F8H6].
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GTX60557 ELISA Image
ELISA analysis of antigen using GTX60557 Fas antibody [4F8H6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60557 FACS Image
FACS analysis of HeLa cells using GTX60557 Fas antibody [4F8H6].
Green : Fas
Red : negative control
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GTX60557 WB Image
WB analysis of HeLa (1), Jurkat (2) cell lysate using GTX60557 Fas antibody [4F8H6].
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GTX60557 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60557 Fas antibody [4F8H6].
Green : Fas
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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REFERENCE
There are currently no references for Fas antibody [4F8H6] (GTX60557). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fas antibody [4F8H6] (GTX60557). Be the first to share your experience with this product.
![Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557](/upload/website/prouct_img/small/GTX60557/GTX60557_20170912_WB_1_w_23061123_994.webp "Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557")
![Anti-Fas antibody [4F8H6] used in ELISA (ELISA). GTX60557](/upload/website/prouct_img/small/GTX60557/GTX60557_20170912_ELISA_w_23061123_655.webp "Anti-Fas antibody [4F8H6] used in ELISA (ELISA). GTX60557")
![Anti-Fas antibody [4F8H6] used in Flow cytometry (FACS). GTX60557](/upload/website/prouct_img/small/GTX60557/GTX60557_20170912_FACS_w_23061123_510.webp "Anti-Fas antibody [4F8H6] used in Flow cytometry (FACS). GTX60557")
![Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557](/upload/website/prouct_img/small/GTX60557/GTX60557_20170912_WB_w_23061123_142.webp "Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557")
![Anti-Fas antibody [4F8H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60557](/upload/website/prouct_img/small/GTX60557/GTX60557_20170912_ICCIF_w_23061123_991.webp "Anti-Fas antibody [4F8H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60557")
![Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557](/upload/website/prouct_img/normal/GTX60557/GTX60557_20170912_WB_1_w_23061123_994.webp "Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557")
![Anti-Fas antibody [4F8H6] used in ELISA (ELISA). GTX60557](/upload/website/prouct_img/normal/GTX60557/GTX60557_20170912_ELISA_w_23061123_655.webp "Anti-Fas antibody [4F8H6] used in ELISA (ELISA). GTX60557")
![Anti-Fas antibody [4F8H6] used in Flow cytometry (FACS). GTX60557](/upload/website/prouct_img/normal/GTX60557/GTX60557_20170912_FACS_w_23061123_510.webp "Anti-Fas antibody [4F8H6] used in Flow cytometry (FACS). GTX60557")
![Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557](/upload/website/prouct_img/normal/GTX60557/GTX60557_20170912_WB_w_23061123_142.webp "Anti-Fas antibody [4F8H6] used in Western Blot (WB). GTX60557")
![Anti-Fas antibody [4F8H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60557](/upload/website/prouct_img/normal/GTX60557/GTX60557_20170912_ICCIF_w_23061123_991.webp "Anti-Fas antibody [4F8H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60557")