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United States (US)

Fas antibody [APO-1-3] (Azide free)

Cat. No. GTX33923

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 APO-1-3

Isotype

 IgG3

Application

 WB, FACS, IP, Functional Assay, Activation

Reactivity

 Human
Package
50 μg ($419)
See all Fas products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
IP Assay dependent
Functional Assay Assay dependent
Activation Assay dependent

Note :

Activation
Induces apoptosis of SKW6.4 cells with a EC50 of 3ng/ml.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

Recognizes human Fas.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Fas.

Purity

??95% by SDS-PAGE.

Conjugation

Unconjugated

RRID

AB_2887729

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fas cell surface death receptor , ALPS1A , APO-1 , APT1 , CD95 , FAS1 , FASTM , TNFRSF6

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Database

Research Area

REFERENCE

Application Reference

Wang W et al. Endocrinology 2016; : en20161464 Induction of amnion epithelial apoptosis by cortisol via tPA/plasmin system.
Application : Functional Assay
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 419