*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis.
0.01M PBS pH7.4, 15 mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
murine L cells transfected with a human Fas/CD95 cDNA.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Fas cell surface death receptor , ALPS1A , APO-1 , APT1 , CD95 , FAS1 , FASTM , TNFRSF6
Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. One of the most studied receptors include the Fas receptor. Apoptosis mediated by both signaling cascades results in activation of a family of cystein proteases known as caspases. Engagement of Fas by its ligand, or by an appropriate antibody, results in the rapid induction of programmed cell death in susceptible cell lines. This process bypasses the usual long sequence of signaling enzymes and immediately activates preexisting caspases.