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Fas antibody [GT1169]

Recombinant antibody KO/KD
Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129
Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129
Anti-Fas antibody [GT1169] used in IHC (Paraffin sections) (IHC-P). GTX09129
Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129
Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129

Cat. No. GTX09129

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1169

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Fas products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan Fas

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fas cell surface death receptor , ALPS1A , APO-1 , APT1 , CD95 , FAS1 , FASTM , TNFRSF6

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129

GTX09129 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fas antibody [GT1169] (GTX09129) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129

GTX09129 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fas antibody [GT1169] (GTX09129) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fas antibody [GT1169] used in IHC (Paraffin sections) (IHC-P). GTX09129

GTX09129 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX09129 Fas antibody [GT1169].
Dlution : 1:100

Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129

GTX09129 WB Image

KO/KD

WB analysis of Wild-type (WT) and Fas knockout (KO) HeLa cell extracts using GTX09129 Fas antibody [GT1169].
Dilution : 1:1000
Loading : 25μg

Anti-Fas antibody [GT1169] used in Western Blot (WB). GTX09129

GTX09129 WB Image

WB analysis of Jurkat and HepG2 whole cell lysates using GTX09129 Fas antibody [GT1169].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399