GeneTex
United States (US)

Fas antibody, Internal

GTX88349_1008_IHC-P_18121411_393.jpg
GTX88349_1007_IHC-P_18121411_577.jpg
GTX88349_2416_WB_18121411_570.jpg

Cat No. GTX88349

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88349-PEP).

Specificity/Sensitivity

This antibody is expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence KTCRKHRKENQGSH, from the internal region of the protein sequence according to NP_000034.1; NP_690610.1; NP_690611.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fas Cell Surface Death Receptor,Alps1A,Apo-1,Apt1,Cd95,Fas1,Fastm,Tnfrsf6,Fas

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES
GTX88349_1008_IHC-P_18121411_393.jpg

GTX88349 IHC-P Image

IHC-P analysis of human small intestine using GTX88349 Fas antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88349_1007_IHC-P_18121411_577.jpg

GTX88349 IHC-P Image

IHC-P analysis of human tonsil using GTX88349 Fas antibody, Internal.
Antigen retrieval : citrate buffer pH 6

GTX88349_2416_WB_18121411_570.jpg

GTX88349 WB Image

WB analysis of Molt-4 cell lysate using GTX88349 Fas antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer