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Fas antibody [ZB4]

Anti-Fas antibody [ZB4] used in Western Blot (WB). GTX13549

Cat No. GTX13549

Host

Mouse

Clonality

Monoclonal

Clone Name

ZB4

Isotype

IgG1

Application

WB, FACS, Blocking, IHC, in vivo

Reactivity

Human
Package
50 μg ($319)

APPLICATION

Application Note

FACS: Use at a concentration of 5 - 20 μg/ml. FuncS: Use at a concentration of 500 ng/ml to block the apoptotic activity other anti-CD95 antibodies. IHC: Use at an assay dependent dilution. WB: Use at a concentration of 5 μg/ml. Predicted molecular weight: 44 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody has been demonstrated to block the apoptotic activity of other anti-CD95 antibodies that are known to induce apoptosis.

PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 10mg/ml Sucrose, PBS. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

Protein A purified
This product has been affinity purified.

Conjugation

Unconjugated

RRID

AB_367890

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fas cell surface death receptor , ALPS1A , APO-1 , APT1 , CD95 , FAS1 , FASTM , TNFRSF6

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-Fas antibody [ZB4] used in Western Blot (WB). GTX13549

GTX13549 WB Image

Western blot analysis of human Fas expression in human Fas transfected cells
Lane 1: anti-human Fas (ZB4)
Lane 2: anti-human Fas (UB2)
Lane 3: anti-human Fas (CH11).

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 319