APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT13D2 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FSCN3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fascin actin-bundling protein 1 , FAN1 , FSCN1 , HSN , SNL , fascin actinbundling protein 1 , p55 , FASCIN 1
Cellular Localization
Cytoplasm,Cytosol,Cytoskeleton,Cell projection,Filopodium,Invadopodium,Microvillus,Cell junction
Background
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
![Anti-Fascin 1 antibody [AT13D2] used in Western Blot (WB). GTX57621](/templates/default/js/lightbox/images/loading.gif "Anti-Fascin 1 antibody [AT13D2] used in Western Blot (WB). GTX57621")
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GTX57621 WB Image
WB analysis of various samples using GTX57621 Fascin 1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : THP-1 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57621 FACS Image
FACS analysis of HeLa cells using GTX57621 Fascin 1 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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GTX57621 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57621 Fascin 1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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REFERENCE
There are currently no references for Fascin 1 antibody [AT13D2] (GTX57621). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fascin 1 antibody [AT13D2] (GTX57621). Be the first to share your experience with this product.
![Anti-Fascin 1 antibody [AT13D2] used in Western Blot (WB). GTX57621](/upload/website/prouct_img/small/GTX57621/GTX57621_20200204_WB_w_23061123_624.webp "Anti-Fascin 1 antibody [AT13D2] used in Western Blot (WB). GTX57621")
![Anti-Fascin 1 antibody [AT13D2] used in Flow cytometry (FACS). GTX57621](/upload/website/prouct_img/small/GTX57621/GTX57621_20200204_FC_w_23061123_429.webp "Anti-Fascin 1 antibody [AT13D2] used in Flow cytometry (FACS). GTX57621")
![Anti-Fascin 1 antibody [AT13D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57621](/upload/website/prouct_img/small/GTX57621/GTX57621_20200204_IF_w_23061123_594.webp "Anti-Fascin 1 antibody [AT13D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57621")
![Anti-Fascin 1 antibody [AT13D2] used in Western Blot (WB). GTX57621](/upload/website/prouct_img/normal/GTX57621/GTX57621_20200204_WB_w_23061123_624.webp "Anti-Fascin 1 antibody [AT13D2] used in Western Blot (WB). GTX57621")
![Anti-Fascin 1 antibody [AT13D2] used in Flow cytometry (FACS). GTX57621](/upload/website/prouct_img/normal/GTX57621/GTX57621_20200204_FC_w_23061123_429.webp "Anti-Fascin 1 antibody [AT13D2] used in Flow cytometry (FACS). GTX57621")
![Anti-Fascin 1 antibody [AT13D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57621](/upload/website/prouct_img/normal/GTX57621/GTX57621_20200204_IF_w_23061123_594.webp "Anti-Fascin 1 antibody [AT13D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57621")