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Fascin 1 antibody [FSCN1/416]

Anti-Fascin 1 antibody [FSCN1/416] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34717
Anti-Fascin 1 antibody [FSCN1/416] used in IHC (Paraffin sections) (IHC-P). GTX34717
Anti-Fascin 1 antibody [FSCN1/416] used in Flow cytometry (FACS). GTX34717
Anti-Fascin 1 antibody [FSCN1/416] used in Western Blot (WB). GTX34717

Cat. No. GTX34717

Host

Mouse

Clonality

Monoclonal

Clone Name

FSCN1/416

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human FSCN1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FAN1 , FSCN1 , HSN , SNL , fascin actinbundling protein 1 , p55 , FASCIN 1 , fascin actin-bundling protein 1

Cellular Localization

Cytoplasm,Cytosol,Cytoskeleton,Cell projection,Filopodium,Invadopodium,Microvillus,Cell junction

Background

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Fascin 1 antibody [FSCN1/416] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34717

GTX34717 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34717 Fascin 1 antibody [FSCN1/416].
Green : Primary antibody
Red : nucleus

Anti-Fascin 1 antibody [FSCN1/416] used in IHC (Paraffin sections) (IHC-P). GTX34717

GTX34717 IHC-P Image

IHC-P analysis of human Hodgkin's lymphoma tissue using GTX34717 Fascin 1 antibody [FSCN1/416].

Anti-Fascin 1 antibody [FSCN1/416] used in Flow cytometry (FACS). GTX34717

GTX34717 FACS Image

FACS analysis of PFA-fixed K562 cells using GTX34717 Fascin 1 antibody [FSCN1/416].
Blue : Primary antibody
Red : Isotype control

Anti-Fascin 1 antibody [FSCN1/416] used in Western Blot (WB). GTX34717

GTX34717 WB Image

WB analysis of HeLa cell lysate using GTX34717 Fascin 1 antibody [FSCN1/416].

REFERENCE

There are currently no references for Fascin 1 antibody [FSCN1/416] (GTX34717). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Fascin 1 antibody [FSCN1/416] (GTX34717). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529