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Fascin 1 antibody [FSCN1/417]

Anti-Fascin 1 antibody [FSCN1/417] used in IHC (Paraffin sections) (IHC-P). GTX34718
Anti-Fascin 1 antibody [FSCN1/417] used in Western Blot (WB). GTX34718
Anti-Fascin 1 antibody [FSCN1/417] used in IHC (Paraffin sections) (IHC-P). GTX34718
Anti-Fascin 1 antibody [FSCN1/417] used in IHC (Paraffin sections) (IHC-P). GTX34718

Cat No. GTX34718

Host Mouse
Clonality Monoclonal
Clone Name FSCN1/417
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkinā€™s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Full length recombinant human FSCN1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fascin Actin-Bundling Protein 1 , Fan1 , Hsn , Snl , P55 , Fscn1

Background

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
Anti-Fascin 1 antibody [FSCN1/417] used in IHC (Paraffin sections) (IHC-P). GTX34718

GTX34718 IHC-P Image

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417)

Anti-Fascin 1 antibody [FSCN1/417] used in Western Blot (WB). GTX34718

GTX34718 WB Image

Western Blot of HeLa Cell Lysate using Fascin-1 Monoclonal Antibody (FSN1/417)

Anti-Fascin 1 antibody [FSCN1/417] used in IHC (Paraffin sections) (IHC-P). GTX34718

GTX34718 IHC-P Image

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417)

Anti-Fascin 1 antibody [FSCN1/417] used in IHC (Paraffin sections) (IHC-P). GTX34718

GTX34718 IHC-P Image

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Fascin-1 Monoclonal Antibody (FSCN1/417)

REFERENCE
There are currently no references for Fascin 1 antibody [FSCN1/417] (GTX34718). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 429