Wild-type (WT) and Fascin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fascin 1 antibody [N2C2], Internal (GTX100511) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100511 diluted at 1:1000