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Fascin 1 antibody [N2C2], Internal

Anti-Fascin 1 antibody [N2C2], Internal used in Western Blot (WB). GTX100511
Anti-Fascin 1 antibody [N2C2], Internal used in Western Blot (WB). GTX100511

Cat No. GTX100511

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fascin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950313

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fascin actin-bundling protein 1 , FAN1 , HSN , SNL , p55

Background

Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.

Database

Research Area

DATA IMAGES

Anti-Fascin 1 antibody [N2C2], Internal used in Western Blot (WB). GTX100511

GTX100511 WB Image

Wild-type (WT) and Fascin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fascin 1 antibody [N2C2], Internal (GTX100511) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Fascin 1 antibody [N2C2], Internal used in Western Blot (WB). GTX100511

GTX100511 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100511 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf