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Fascin 1 antibody

KO/KD
Anti-Fascin 1 antibody used in Western Blot (WB). GTX117805
Anti-Fascin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117805
Anti-Fascin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117805
Anti-Fascin 1 antibody used in Western Blot (WB). GTX117805

Cat. No. GTX117805

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Fascin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fascin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885420

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fascin actin-bundling protein 1 , FAN1 , HSN , SNL , p55

Background

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Fascin 1 antibody used in Western Blot (WB). GTX117805

GTX117805 WB Image

KO/KD

Wild-type (WT) and Fascin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fascin 1 antibody (GTX117805) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fascin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117805

GTX117805 ICC/IF Image

FSCN1 antibody detects FSCN1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 100% MeOH for 5 min.
Green: FSCN1 protein stained by FSCN1 antibody (GTX117805) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Fascin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117805

GTX117805 IHC-P Image

Fascin 1 antibody detects Fascin 1 protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
Fascin 1 antibody (GTX117805) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fascin 1 antibody used in Western Blot (WB). GTX117805

GTX117805 WB Image

Fascin 1 antibody detects Fascin 1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
Fascin 1 antibody (GTX117805) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169