APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fascin actin-bundling protein 1 , FAN1 , HSN , SNL , p55
Cellular Localization
Cytoplasm, cytosol,Cytoplasm, cytoskeleton,Cell junction
Background
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
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GTX55616 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55616 Fascin 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX55616 ICC/IF Image
ICC/IF analysis of C6 cells using GTX55616 Fascin 1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX55616 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX55616 Fascin 1 antibody.
Dilution : 1:100
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GTX55616 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX55616 Fascin 1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX55616 WB Image
WB analysis of various sample lysates using GTX55616 Fascin 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
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REVIEW
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