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Fatty Acid Synthase antibody [GT325]

KO/KD Comparison Orthogonal
Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629
Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629
Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629
Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629
Anti-Fatty Acid Synthase antibody [GT325] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629629
Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629

Cat. No. GTX629629

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT325

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fatty Acid Synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Positive Control

Neuro2A , GL261 , BCL-1 , PC-12 , Rat-2 , 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm , Melanosome

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629

GTX629629 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325] (GTX629629) diluted at 1:500.

Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629

GTX629629 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Fatty Acid Synthase antibody [GT325] (GTX629629) diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629

GTX629629 WB Image

Orthogonal

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325] (GTX629629) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629

GTX629629 WB Image

Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
Fatty Acid Synthase antibody (GTX629629) dilution: 1:5000

Anti-Fatty Acid Synthase antibody [GT325] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629629

GTX629629 ICC/IF Image

Fatty Acid Synthase antibody [GT325] detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [GT325] (GTX629629) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Fatty Acid Synthase antibody [GT325] used in Western Blot (WB). GTX629629

GTX629629 WB Image

Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
5 % SDS-PAGE
Fatty Acid Synthase antibody (GTX629629) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations