APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888152
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fatty acid synthase , FAS , OA-519 , SDR27X1
Cellular Localization
Cytoplasm , Melanosome
Background
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761](/templates/default/js/lightbox/images/loading.gif "Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761")
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GTX629761 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT5210] (GTX629761) diluted at 1:500.
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GTX629761 ICC/IF Image
Fatty Acid Synthase antibody [GT5210] detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [GT5210] (GTX629761) diluted at 1:200.
Blue: Hoechst 33342 staining.
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GTX629761 WB Image
Fatty Acid Synthase antibody [GT5210] detects Fatty Acid Synthase protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
Fatty Acid Synthase antibody [GT5210] (GTX629761) dilution: 1:1000
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REFERENCE
There are currently no references for Fatty Acid Synthase antibody [GT5210] (GTX629761). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fatty Acid Synthase antibody [GT5210] (GTX629761). Be the first to share your experience with this product.
![Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761](/upload/website/prouct_img/small/GTX629761/GTX629761_41435_20160616_WB_shRNA_watermark_w_23061202_982.webp "Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761")
![Anti-Fatty Acid Synthase antibody [GT5210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629761](/upload/website/prouct_img/small/GTX629761/GTX629761_41435_20170913_IFA_w_23061202_178.webp "Anti-Fatty Acid Synthase antibody [GT5210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629761")
![Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761](/upload/website/prouct_img/small/GTX629761/GTX629761_41435_WB_w_23061202_952.webp "Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761")
![Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761](/upload/website/prouct_img/normal/GTX629761/GTX629761_41435_20160616_WB_shRNA_watermark_w_23061202_982.webp "Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761")
![Anti-Fatty Acid Synthase antibody [GT5210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629761](/upload/website/prouct_img/normal/GTX629761/GTX629761_41435_20170913_IFA_w_23061202_178.webp "Anti-Fatty Acid Synthase antibody [GT5210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629761")
![Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761](/upload/website/prouct_img/normal/GTX629761/GTX629761_41435_WB_w_23061202_952.webp "Anti-Fatty Acid Synthase antibody [GT5210] used in Western Blot (WB). GTX629761")