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Fatty Acid Synthase antibody [GT556]

Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762
Anti-Fatty Acid Synthase antibody [GT556] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629762
Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762
Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762
Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762

Cat No. GTX629762

Host

Mouse

Clonality

Monoclonal

Clone Name

GT556

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm , Melanosome

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762

GTX629762 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:500.

Anti-Fatty Acid Synthase antibody [GT556] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629762

GTX629762 ICC/IF Image

Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-Fatty Acid Synthase antibody [GT556] used in Western Blot (WB). GTX629762

GTX629762 WB Image

Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
Fatty Acid Synthase antibody [GT556] (GTX629762) dilution: 1:1000

Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762

GTX629762 IHC-P Image

Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX629762

GTX629762 IHC-P Image

Fatty Acid Synthase antibody [GT556] detects Fatty Acid Synthase protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Fatty Acid Synthase antibody [GT556] (GTX629762) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169