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Fatty Acid Synthase antibody [HL2161]

Recombinant antibody KO/KD Comparison Orthogonal
Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139
Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139
Anti-Fatty Acid Synthase antibody [HL2161] used in IHC (Paraffin sections) (IHC-P). GTX638139
Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139
Anti-Fatty Acid Synthase antibody [HL2161] used in IHC (Paraffin sections) (IHC-P). GTX638139
Anti-Fatty Acid Synthase antibody [HL2161] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638139
Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139
Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139

Cat. No. GTX638139

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2161

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fatty Acid Synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human Fatty Acid Synthase

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm,Melanosome

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139

GTX638139 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139

GTX638139 WB Image

Comparison

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Fatty Acid Synthase antibody [HL2161] used in IHC (Paraffin sections) (IHC-P). GTX638139

GTX638139 IHC-P Image

Fatty Acid Synthase antibody [HL2161] detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139

GTX638139 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [HL2161] used in IHC (Paraffin sections) (IHC-P). GTX638139

GTX638139 IHC-P Image

Fatty Acid Synthase antibody [HL2161] detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [HL2161] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638139

GTX638139 ICC/IF Image

Fatty Acid Synthase antibody [HL2161] detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Fatty Acid Synthase stained by Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139

GTX638139 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [HL2161] used in Western Blot (WB). GTX638139

GTX638139 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2161] (GTX638139) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations