Fatty Acid Synthase antibody [N1], N-term

Name: Fatty Acid Synthase antibody [N1], N-term
Brand: GeneTex
SKU: GTX109833
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunoprecipitation (IP). GTX109833

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109833

Cat No. GTX109833

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all Fatty Acid Synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:1000

Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Positive Control

Molt-4 , mouse brain , PC-12 , Rat2 , 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm , Melanosome

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

Database

Gene ID: 2194 FASN
UniProt: P49327 FASN

Research Area

DATA IMAGES

GTX109833 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109833 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian carainoma, using Fatty Acid Synthase(GTX109833) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109833 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109833 WB Image

Fatty Acid Synthase antibody [N1], N-term detects FASN protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
Fatty Acid Synthase antibody [N1], N-term (GTX109833) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109833 IP Image

FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FASN by 2.5 μg of FASN antibody (GTX109833) 5% SDS-PAGE The immunoprecipitated FASN protein was detected by FASN antibody (GTX109833) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX109833 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109833 ICC/IF Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109833 IHC-P Image

GTX109833 WB Image

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

REFERENCE

Application Reference

You BJ et al. Sci Rep 2019; 9 (1) : 3306 Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase.

Application : WB

Reactivity : Human

Ezzeddini R et al. Life Sci 2019; 224 : 169-176 Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma.

Application : WB

Reactivity : Human

Shimada M et al. Nutr Res 2017; 47 : 21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.

Application : WB

Reactivity : Rat

Gui Y et al. Cardiovasc Res 2016; A novel small molecule liver X receptor transcriptional regulator, nagilactone B, suppresses atherosclerosis in apoE-deficient mice.

Application : WB

Reactivity : Mouse

Jhuang HJ et al. Oncotarget 2015; 6 (10) : 7788-803 Gluconeogenesis, lipogenesis, and HBV replication are commonly regulated by PGC-1α-dependent pathway.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW