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Fatty Acid Synthase antibody [N1], N-term

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunoprecipitation (IP). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109833

Cat No. GTX109833

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Positive Control

Molt-4 , mouse brain , PC-12 , Rat2 , 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950258

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm , Melanosome

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian carainoma, using Fatty Acid Synthase(GTX109833) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Fatty Acid Synthase antibody [N1], N-term detects FASN protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
Fatty Acid Synthase antibody [N1], N-term (GTX109833) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunoprecipitation (IP). GTX109833

GTX109833 IP Image

FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FASN by 2.5 μg of FASN antibody (GTX109833) 5% SDS-PAGE The immunoprecipitated FASN protein was detected by FASN antibody (GTX109833) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109833

GTX109833 ICC/IF Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Fatty Acid Synthase antibody [N1], N-term Cat No. GTX109833

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 6 users reviews)
Date : Anonymous submitted on 06-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 30 μg
Blocking : 5% milk in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:1000, RT°C, 1Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 35
Blocking : 5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 35
Blocking : 5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Date : Anonymous submitted on 15-Jul-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 40 μg
Blocking : 5% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019.PMID: 31614639
Date : Anonymous submitted on 04-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : liver tissue
Amount used : 40μg
Blocking : 5% skim milk, 20°C, 1Hr
Primary Antibody : 1:6000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169