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Fatty Acid Synthase antibody [N1], N-term

KO/KD Orthogonal
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunoprecipitation (IP). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

Cat. No. GTX109833

Reference 14
6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fatty Acid Synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Positive Control

Mouse brain , Molt-4 , PC-12 , Rat2 , 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950258

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm , Melanosome

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109833 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109833

GTX109833 ICC/IF Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian carainoma, using Fatty Acid Synthase(GTX109833) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Immunoprecipitation (IP). GTX109833

GTX109833 IP Image

FASN antibody immunoprecipitates FASN protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of FASN protein expressing HeLa cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of FASN by 2.5 μg of FASN antibody (GTX109833) 5% SDS-PAGE The immunoprecipitated FASN protein was detected by FASN antibody (GTX109833) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Fatty Acid Synthase antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX109833

GTX109833 IHC-P Image

Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833

GTX109833 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019. PMID: 31614639

REFERENCE

Application Reference

Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
Application : WB
Giuliani A et al. Antioxidants (Basel) 2023; 12 (11) Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation.
Tsao CH et al. Front Med (Lausanne) 2022; 9 : 1008200 Dual-tracer positron emission tomography/computed tomography as an imaging probe of de novo lipogenesis in preclinical models of hepatocellular carcinoma.
Application : WB
Reactivity : Human
Ceja-Galicia ZA et al. Antioxidants (Basel) 2022; 11 (11) Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial β-Oxidation and Lipid Metabolism in Kidney and Liver.
Lu Y et al. nanotoday 2022; 43 : 101416 Reactivation of dysfunctional dendritic cells by a stress-relieving nanosystem resets anti-tumor immune landscape.
Show More +
Submit a Reference

REVIEW

Fatty Acid Synthase antibody [N1], N-term Cat. No. GTX109833

Rating
( Average 5 based on 6 users reviews)
Application
Western Blot(WB)
( Average 5 based on 6 users reviews)
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 18-Apr-2023
Score :
Application Tested : WB
Sample Species : Rat
Sample : adipose tissue
Amount used : 50μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 06-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 30 μg
Blocking : 5% milk in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:1000, RT°C, 1Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 35
Blocking : 5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 35
Blocking : 5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 15-Jul-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 40 μg
Blocking : 5% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-Fatty Acid Synthase antibody [N1], N-term used in Western Blot (WB). GTX109833
Date : Anonymous submitted on 04-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : liver tissue
Amount used : 40μg
Blocking : 5% skim milk, 20°C, 1Hr
Primary Antibody : 1:6000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
Application : WB
Giuliani A et al. Antioxidants (Basel) 2023; 12 (11) Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation.
Tsao CH et al. Front Med (Lausanne) 2022; 9:1008200 Dual-tracer positron emission tomography/computed tomography as an imaging probe of de novo lipogenesis in preclinical models of hepatocellular carcinoma.
Application : WB
Reactivity : Human
Ceja-Galicia ZA et al. Antioxidants (Basel) 2022; 11 (11) Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial β-Oxidation and Lipid Metabolism in Kidney and Liver.
Lu Y et al. nanotoday 2022; 43:101416 Reactivation of dysfunctional dendritic cells by a stress-relieving nanosystem resets anti-tumor immune landscape.
Park S et al. Exp Mol Med 2021; 53 (7):1159-1169 Loss of Acot12 contributes to NAFLD independent of lipolysis of adipose tissue.
Application : IHC-Fr
Reactivity : Mouse
Raineri A et al. Molecules. 2021; 26 (16):5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.
Application : WB
Reactivity : Mouse
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Garc?a-Arroyo FE et al. Biomolecules 2019; 9 (10) Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats.
You BJ et al. Sci Rep 2019; 9 (1):3306 Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase.
Application : WB
Reactivity : Human
Ezzeddini R et al. Life Sci 2019; 224:169-176 Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma.
Application : WB
Reactivity : Human
Shimada M et al. Nutr Res 2017; 47:21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Gui Y et al. Cardiovasc Res 2016; A novel small molecule liver X receptor transcriptional regulator, nagilactone B, suppresses atherosclerosis in apoE-deficient mice.
Application : WB
Reactivity : Mouse
Jhuang HJ et al. Oncotarget 2015; 6 (10):7788-803 Gluconeogenesis, lipogenesis, and HBV replication are commonly regulated by PGC-1α-dependent pathway.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169