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Fatty Acid Synthase antibody [N1N2], N-term

KO/KD Orthogonal
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470
Anti-Fatty Acid Synthase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110470

Cat. No. GTX110470

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Fatty Acid Synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

Positive Control

Molt-4 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950257

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm , Melanosome

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110470

GTX110470 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase(GTX110470) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110470

GTX110470 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OECM1 xenograft, using Fatty Acid Synthase(GTX110470) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470

GTX110470 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
5% SDS PAGE
GTX110470 diluted at 1:1000

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470

GTX110470 WB Image

Orthogonal

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110470

GTX110470 IHC-P Image

Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470

GTX110470 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:3000.

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110470

GTX110470 ICC/IF Image

Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX110470

GTX110470 IP Image

Immunoprecipitation of Fatty Acid Synthase protein from HeLa whole cell extracts using 5 μg of Fatty Acid Synthase antibody [N1N2], N-term (GTX110470).
Western blot analysis was performed using Fatty Acid Synthase antibody [N1N2], N-term (GTX110470).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in Western Blot (WB). GTX110470

GTX110470 WB Image

Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody (GTX110470) diluted by 1:1000.

Anti-Fatty Acid Synthase antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110470

GTX110470 IHC-P Image

Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

You BJ et al. Chem Res Toxicol 2019; 32 (2) : 255-264
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Fatty Acid Synthase antibody [N1N2], N-term (GTX110470). Be the first to share your experience with this product.
Submit a Review
Application Reference
You BJ et al. Chem Res Toxicol 2019; 32 (2):255-264
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169