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Fatty Acid Synthase antibody

Anti-Fatty Acid Synthase antibody used in Western Blot (WB). GTX32598
Anti-Fatty Acid Synthase antibody used in IHC (Paraffin sections) (IHC-P). GTX32598
Anti-Fatty Acid Synthase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32598
Anti-Fatty Acid Synthase antibody used in Immunoprecipitation (IP). GTX32598

Cat No. GTX32598

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP 1:50-1:100
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human FASN (NP_004095.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fatty acid synthase , FAS , OA-519 , SDR27X1

Cellular Localization

Cytoplasm

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Fatty Acid Synthase antibody used in Western Blot (WB). GTX32598

GTX32598 WB Image

WB analysis of various sample lysates using GTX32598 Fatty Acid Synthase antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Fatty Acid Synthase antibody used in IHC (Paraffin sections) (IHC-P). GTX32598

GTX32598 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX32598 Fatty Acid Synthase antibody.
Dilution : 1:200

Anti-Fatty Acid Synthase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32598

GTX32598 ICC/IF Image

ICC/IF analysis of A549 cells using GTX32598 Fatty Acid Synthase antibody.
Blue : DAPI

Anti-Fatty Acid Synthase antibody used in Immunoprecipitation (IP). GTX32598

GTX32598 IP Image

IP analysis of 293T cells using GTX32598 Fatty Acid Synthase antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329