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Fc epsilon R1 alpha antibody [CRA1] (Biotin)

Anti-Fc epsilon R1 alpha antibody [CRA1] (Biotin) used in Flow cytometry (FACS). GTX00849

Cat No. GTX00849

Host

Mouse

Clonality

Monoclonal

Clone Name

CRA1

Isotype

IgG2b

Application

WB, IHC-P, IHC-Fr, FACS, ELISA, Functional Assay

Reactivity

Human, House musk shrew
Package
50 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB ~1 μg /ml
IHC-P ~1 μg /ml
IHC-Fr Assay dependent
FACS 1-5 μg /ml
ELISA Assay dependent
Functional Assay Assay dependent

Note :

Functional Assay
Titration of IgE-bound fraction of the Fc epsilon R1 alpha using clone CRA1 and CRA2 antibodies.

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Specificity/Sensitivity

The CRA1 (AER37) monoclonal antibody reacts with the Fc epsilon R1 alpha subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete withIgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on Fc epsilon R1 alpha, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound Fc epsilon R1 alpha.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS (pH 7.4), 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant extracellular protein of human Fc epsilonR1alpha; (corresponding to amino acids Met-26-197, where signal peptide is 1-25)
Epitope: 26-110 amino acids

Purification

Purified IgG
From culture supernant

Conjugation

Biotin
[Biotin]/[IgG] = 8.9; Lot dependent

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fc fragment of IgE receptor Ia , FCE1A , FCER1A , FcERI , Fc epsilon R1 alpha

Cellular Localization

Cell membrane

Background

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Fc epsilon R1 alpha antibody [CRA1] (Biotin) used in Flow cytometry (FACS). GTX00849

GTX00849 FACS Image

FACS analysis of U266 cells (pre-incubated with recombinant soluble Fc epsilon R1 alpha protein) using GTX00849 Fc epsilon R1 alpha antibody [CRA1] (Biotin).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 369