*Optimal dilutions/concentrations should be determined by the researcher.
|~1 μg /ml
Titration of IgE-bound fraction of the Fc epsilon R1 alpha using clone CRA1 and CRA2 antibodies.
Not tested in other applications.
The CRA1 (AER37) monoclonal antibody reacts with the Fc epsilon R1 alpha subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete withIgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on Fc epsilon R1 alpha, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound Fc epsilon R1 alpha.
Filter-sterilized PBS (pH 7.4), 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant extracellular protein of human Fc epsilonR1alpha; (corresponding to amino acids Met-26-197, where signal peptide is 1-25)
Epitope: 110-197 amino acids
From culture supernant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Fc fragment of IgE receptor Ia , FCE1A , FCER1A , FcERI , Fc epsilon R1 alpha
The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]