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Fc epsilon R1 alpha antibody [CRA2]

Anti-Fc epsilon R1 alpha antibody [CRA2] used in Flow cytometry (FACS). GTX00712
Anti-Fc epsilon R1 alpha antibody [CRA2] used in IHC (Frozen sections) (IHC-Fr). GTX00712
Anti-Fc epsilon R1 alpha antibody [CRA2] used in Western Blot (WB). GTX00712

Cat No. GTX00712

Host

Mouse

Clonality

Monoclonal

Clone Name

CRA2

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, Activation, Inhibition assay

Reactivity

Human
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB ~1 μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Activation Assay dependent
Inhibition assay Assay dependent

Note :

Activation
It shows the stimulation of serotonin release from human platelets.

Inhibition assay
It shows the inhibition of binding of IgE with Fc epsilonR1alpha and titration of IgE-bound fraction of the Fc epsilonR1alpha (using CRA1 and CRA2 antibodies).

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant extracellular protein of human Fc epsilonR1alpha; (corresponding to amino acids Met-26-197, where signal peptide is 1-25).
Epitope: 110-197 amino acids

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Fc fragment of IgE receptor Ia , FCE1A , FcERI

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Fc epsilon R1 alpha antibody [CRA2] used in Flow cytometry (FACS). GTX00712

GTX00712 FACS Image

FACS analysis of CHO cells overexpressed human Fc epsilon R1 alpha protein using GTX00712 Fc epsilon R1 alpha antibody [CRA2].

Anti-Fc epsilon R1 alpha antibody [CRA2] used in IHC (Frozen sections) (IHC-Fr). GTX00712

GTX00712 IHC-Fr Image

IHC-Fr analysis of human atopic dermatis lesional skin tissue section using GTX00712 Fc epsilon R1 alpha antibody [CRA2].
Fixation : Aceton

Anti-Fc epsilon R1 alpha antibody [CRA2] used in Western Blot (WB). GTX00712

GTX00712 WB Image

WB analysis of E. coli cells overexpressing truncated extracelluar domains of Fc epsilon R1 alpha protein (maltose binding protein tagged) using GTX00712 Fc epsilon R1 alpha antibody [CRA2].
Lane 1 : MalE-LacZ (control)
Lane 2 : 26-197
Lane 3 : 68-197
Lane 4 : 26-109
Lane 5 : 26-153
Lane 6 : 68-153

REFERENCE

There are currently no references for Fc epsilon R1 alpha antibody [CRA2] (GTX00712). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Fc epsilon R1 alpha antibody [CRA2] (GTX00712). Be the first to share your experience with this product.
Package List Price ($)
$ 359