Fc epsilon R1 alpha antibody [CRA2]

Cat. No. GTX00712

Host	Mouse
Clonality	Monoclonal
Clone Name	CRA2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, Activation, Neutralizing/Inhibition
Reactivity	Human
Package	100 μg ($399)

See all Fc epsilon R1 alpha products

Datasheet
Document

View product citations for antibody GTX00712 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	~1 μg/ml
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	Assay dependent
Activation	Assay dependent
Neutralizing/Inhibition	Assay dependent
Note : Activation It shows the stimulation of serotonin release from human platelets. It shows the inhibition of binding of IgE with Fc epsilonR1alpha and titration of IgE-bound fraction of the Fc epsilonR1alpha (using CRA1 and CRA2 antibodies).

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant extracellular protein of human Fc epsilonR1alpha; (corresponding to amino acids Met-26-197, where signal peptide is 1-25).
Epitope: 110-197 amino acids

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgE receptor Ia , FCE1A , FcERI

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Database

Gene ID: 2205 FCER1A
UniProt: P12319 FCER1A

Research Area

DATA IMAGES

GTX00712 FACS Image

FACS analysis of CHO cells overexpressed human Fc epsilon R1 alpha protein using GTX00712 Fc epsilon R1 alpha antibody [CRA2].

GTX00712 WB Image

WB analysis of E. coli cells overexpressing truncated extracelluar domains of Fc epsilon R1 alpha protein (maltose binding protein tagged) using GTX00712 Fc epsilon R1 alpha antibody [CRA2].
Lane 1 : MalE-LacZ (control)
Lane 2 : 26-197
Lane 3 : 68-197
Lane 4 : 26-109
Lane 5 : 26-153
Lane 6 : 68-153

GTX00712 IHC-Fr Image

IHC-Fr analysis of human atopic dermatis lesional skin tissue section using GTX00712 Fc epsilon R1 alpha antibody [CRA2].
Fixation : Aceton

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf

Package

List Price ($)

100 μg

$ 399

Qty