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Fc epsilon R1 alpha antibody

Anti-Fc epsilon R1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX04729
Anti-Fc epsilon R1 alpha antibody used in Western Blot (WB). GTX04729
Anti-Fc epsilon R1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX04729
Anti-Fc epsilon R1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX04729
Anti-Fc epsilon R1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04729

Cat. No. GTX04729

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Fc epsilon R1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human FCER1A(Accession P12319), corresponding to amino acid residues Q227-N257.

Purification


From serum

Purity

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FCE1A,FCER1A,Fc fragment of IgE receptor Ia,FcERI,Fc epsilon R1 alpha

Cellular Localization

Cell membrane

Background

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Fc epsilon R1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX04729

GTX04729 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04729 Fc epsilon R1 alpha antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-Fc epsilon R1 alpha antibody used in Western Blot (WB). GTX04729

GTX04729 WB Image

WB analysis of rat liver tissue lysate using GTX04729 Fc epsilon R1 alpha antibody. The lane on the left was treated with blocking peptide.

Anti-Fc epsilon R1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX04729

GTX04729 IHC-P Image

IHC-P analysis of human kidney cancer and adjacent normal tissue using GTX04729 Fc epsilon R1 alpha antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-Fc epsilon R1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX04729

GTX04729 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX04729 Fc epsilon R1 alpha antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-Fc epsilon R1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04729

GTX04729 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04729 Fc epsilon R1 alpha antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 399