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Ferredoxin 1 + Ferredoxin 2 antibody

Anti-Ferredoxin 1 +  Ferredoxin 2 antibody used in Western Blot (WB). GTX00911
Anti-Ferredoxin 1 +  Ferredoxin 2 antibody used in Western Blot (WB). GTX00911

Cat No. GTX00911

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Arabidopsis thaliana, Zea mays
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Specificity/Sensitivity

It reacts with plant Fd1 and Fd2 isoproteins including those of Maize and Arabidopsis.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zea mays

Immunogen

Purified recombinant Maize Fd1 protein (full-size, no Tag)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Plastid,Chloroplast

Database

DATA IMAGES

Anti-Ferredoxin 1 +  Ferredoxin 2 antibody used in Western Blot (WB). GTX00911

GTX00911 WB Image

WB analysis of various samples using GTX00911 Ferredoxin 1 + Ferredoxin 2 antibody. Molecular mass of Maize Fds are about 12kDa, but migrates at the position around 15 kDa on SDS-PAGE.
Lane 1 : Recombinant Maize Fd1 protein
Lane 2 : Arabidopsis leaf extract (10 μg)
Lane 3 : Maize leaf extract (10 μg)
Dilution : 1:1000

Anti-Ferredoxin 1 +  Ferredoxin 2 antibody used in Western Blot (WB). GTX00911

GTX00911 WB Image

WB analysis of various samples using GTX00911 Ferredoxin 1 + Ferredoxin 2 antibody. The Maize leaf type specific antibody, anti-Ferredoxin-1 antibody also specifically reacts with Arabidopsis leaf type ferredoxins, 1 and 2 isoproteins.
Lane 1 : Recombinant At-Feredoxin-1 (200 nmol)
Lane 2 : Recombinant At-Ferredoxin-2 (200 nmol)
Lane 3 : Recombinant At-Ferredoxin-3 (200 nmol)
Lane 4 : Recombinant At-Ferredoxin-4 (20 nmol)
Lane 5 : Leaf extract of Arabidopsis, soluble fraction with 70% saturated ammonium sulfate
Lane 6 : Leaf extract of Arabidopsis, insoluble fraction with 70% saturated ammonium sulfate
Lane 7 : Root extract of Arabidopsis

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 369