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Ferritin Heavy Chain antibody [N1C3]

Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733
Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733
Anti-Ferritin Heavy Chain antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101733
Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733
Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733
Anti-Ferritin Heavy Chain antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101733
Anti-Ferritin Heavy Chain antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101733
Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733

Cat No. GTX101733

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

human liver , mouse liver , rat liver , HCT116

Predict Reactivity

Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ferritin Heavy Chain. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ferritin heavy chain 1 , FHC , FTH , FTHL6 , HFE5 , PIG15 , PLIF

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733

GTX101733 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733

GTX101733 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Anti-Ferritin Heavy Chain antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101733

GTX101733 ICC/IF Image

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733

GTX101733 WB Image

Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733

GTX101733 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Anti-Ferritin Heavy Chain antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101733

GTX101733 IHC-P Image

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ferritin Heavy Chain antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101733

GTX101733 IHC-P Image

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ferritin Heavy Chain antibody [N1C3] used in Western Blot (WB). GTX101733

GTX101733 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

REFERENCE
There are currently no references for Ferritin Heavy Chain antibody [N1C3] (GTX101733). Be the first to share your publications with this product.
REVIEW

Ferritin Heavy Chain antibody [N1C3] Cat No. GTX101733

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 15-Jul-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : liver tissue lysate
Amount used : 20 μg
Blocking : 5% Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169