GeneTex
United States (US)

Ferritin Heavy Chain antibody [N1C3]

GTX101733_42529_20160908_WB_19011506_316.jpg
GTX101733_42592_20161123_IFA_19011506_100.jpg
GTX101733_42529_20160728_WB_H_liver_19011506_576.jpg
GTX101733_42529_20160714_WB_R_liver_19011506_968.jpg
GTX101733_42529_20160823_IHC-P_M_19011506_972.jpg
GTX101733_42529_20160823_IHC-P_R_19011506_277.jpg
GTX101733_42529_20160714_WB_M_liver_19011506_917.jpg
GTX101733_42592_20160908_WB_19030801_749.jpg

Cat No. GTX101733

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

human liver , mouse liver , rat liver , HCT116

Predict Reactivity

Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ferritin Heavy Chain. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ferritin Heavy Chain 1,Fhc,Fth,Fthl6,Hfe5,Pig15,Plif,Fth1

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101733_42529_20160908_WB_19011506_316.jpg

GTX101733 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

GTX101733_42592_20161123_IFA_19011506_100.jpg

GTX101733 ICC/IF Image

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101733_42529_20160728_WB_H_liver_19011506_576.jpg

GTX101733 WB Image

Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

GTX101733_42529_20160714_WB_R_liver_19011506_968.jpg

GTX101733 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

GTX101733_42529_20160823_IHC-P_M_19011506_972.jpg

GTX101733 IHC-P Image

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101733_42529_20160823_IHC-P_R_19011506_277.jpg

GTX101733 IHC-P Image

Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101733_42529_20160714_WB_M_liver_19011506_917.jpg

GTX101733 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

GTX101733_42592_20160908_WB_19030801_749.jpg

GTX101733 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] (GTX101733) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169