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Ferritin Heavy Chain antibody

Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014
Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014
Anti-Ferritin Heavy Chain antibody used in Western Blot (WB). GTX54014
Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014
Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014
Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014
Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014
Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014
Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014

Cat. No. GTX54014

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Ferritin Heavy Chain products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human FTH1 (NP_002023.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887832

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ferritin heavy chain 1 , FHC , FTH , FTHL6 , HFE5 , PIG15 , PLIF

Background

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014

GTX54014 IHC-P Image

IHC-P analysis of rat liver tissue using GTX54014 Ferritin Heavy Chain antibody.
Dilution : 1:100

Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014

GTX54014 ICC/IF Image

ICC/IF analysis of A549 cells using GTX54014 Ferritin Heavy Chain antibody.
Blue : DAPI
Dilution : 1:100

Anti-Ferritin Heavy Chain antibody used in Western Blot (WB). GTX54014

GTX54014 WB Image

WB analysis of mouse heart tissue lysate using GTX54014 Ferritin Heavy Chain antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014

GTX54014 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX54014 Ferritin Heavy Chain antibody.
Dilution : 1:100

Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014

GTX54014 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54014 Ferritin Heavy Chain antibody.
Blue : DAPI
Dilution : 1:100

Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014

GTX54014 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54014 Ferritin Heavy Chain antibody.
Blue : DAPI
Dilution : 1:100

Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014

GTX54014 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54014 Ferritin Heavy Chain antibody.
Dilution : 1:100

Anti-Ferritin Heavy Chain antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54014

GTX54014 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54014 Ferritin Heavy Chain antibody.
Blue : DAPI
Dilution : 1:100

Anti-Ferritin Heavy Chain antibody used in IHC (Paraffin sections) (IHC-P). GTX54014

GTX54014 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX54014 Ferritin Heavy Chain antibody.
Dilution : 1:100

REFERENCE

Application Reference

Chekhun VF et al. Exp Oncol 2017; 39 (1) : 25-29 Artemisinin modulating effect on human breast cancer cell lines with different sensitivity to cytostatics.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399