APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.1-0.3μg/ml |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88496-PEP).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS pH7.3, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GEYLFERLTLKHD, from the C Terminus of the protein sequence according to NP_000137.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ferritin light chain , LFTD , NBIA3
Background
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX88496 ELISA Image
ELISA analysis of Ferritin Light Chain protein using GTX88496 Ferritin Light Chain antibody, C-term.
Capture : GTX112943 (5μg/ml)
Detection : GTX88496 (1.5μg/ml)
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GTX88496 WB Image
WB analysis of human cerebellum (A), liver (B), placenta (C), mouse brain (D) and rat brain (E) lysates using GTX88496 Ferritin Light Chain antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88496 IHC-P Image
IHC-P analysis of human brain cortex using GTX88496 Ferritin Light Chain antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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REFERENCE
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REVIEW
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