GeneTex
United States (US)

Ferritin Light Chain antibody, C-term

GTX88496_935_IHC-P_18122102_352.jpg
GTX88496_18_ELISA_18122102_365.jpg
GTX88496_2272_WB_18122102_388.jpg

Cat No. GTX88496

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA, sELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P Assay dependent
ELISA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture : GTX112943 , Detection : GTX88496

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88496-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GEYLFERLTLKHD, from the C Terminus of the protein sequence according to NP_000137.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ferritin Light Chain,Lftd,Nbia3,Ftl

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88496_935_IHC-P_18122102_352.jpg

GTX88496 IHC-P Image

IHC-P analysis of human brain cortex using GTX88496 Ferritin Light Chain antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88496_18_ELISA_18122102_365.jpg

GTX88496 ELISA Image

ELISA analysis of Ferritin Light Chain protein using GTX88496 Ferritin Light Chain antibody, C-term.
Capture : GTX112943 (5μg/ml)
Detection : GTX88496 (1.5μg/ml)

GTX88496_2272_WB_18122102_388.jpg

GTX88496 WB Image

WB analysis of human cerebellum (A), liver (B), placenta (C), mouse brain (D) and rat brain (E) lysates using GTX88496 Ferritin Light Chain antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329