Ferritin Light Chain antibody, C-term

Cat No. GTX88496

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA, sELISA
Reactivity	Human, Mouse, Rat

See all Ferritin Light Chain products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.1-0.3μg/ml
IHC-P	Assay dependent
ELISA	Assay dependent
sELISA	Assay dependent
Note : sELISA Capture : GTX112943 , Detection : GTX88496

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88496-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GEYLFERLTLKHD, from the C Terminus of the protein sequence according to NP_000137.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NBIA3 Antibody , FTL Antibody

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2512
UniProt: P02792

Research Area

DATA IMAGES

GTX88496 IHC-P Image

IHC-P analysis of human brain cortex using GTX88496 Ferritin Light Chain antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88496 ELISA Image

ELISA analysis of Ferritin Light Chain protein using GTX88496 Ferritin Light Chain antibody, C-term.
Capture : GTX112943 (5μg/ml)
Detection : GTX88496 (1.5μg/ml)

GTX88496 WB Image

WB analysis of human cerebellum (A), liver (B), placenta (C), mouse brain (D) and rat brain (E) lysates using GTX88496 Ferritin Light Chain antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package	List Price ($)
100 μg	$ 329
Qty

Package

List Price ($)

100 μg

$ 329

Qty