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Ferritin Light Chain antibody [FTL/1388]

Anti-Ferritin Light Chain antibody [FTL/1388] used in IHC (Paraffin sections) (IHC-P). GTX34723
Anti-Ferritin Light Chain antibody [FTL/1388] used in IHC (Paraffin sections) (IHC-P). GTX34723

Cat No. GTX34723

Host Mouse
Clonality Monoclonal
Clone Name FTL/1388
Isotype IgG2a
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.1-0.2ug/ml
IHC-P 0.1-0.2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Specificity/Sensitivity

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human FTL protein fragment (aa 38-165) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ferritin Light Chain,Lftd,Nbia3,Ftl

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Ferritin Light Chain antibody [FTL/1388] used in IHC (Paraffin sections) (IHC-P). GTX34723

GTX34723 IHC-P Image

Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1388).

Anti-Ferritin Light Chain antibody [FTL/1388] used in IHC (Paraffin sections) (IHC-P). GTX34723

GTX34723 IHC-P Image

Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1388).

Package List Price ($)
$ 329