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Ferritin Light Chain antibody [N1C3-3]

Anti-Ferritin Light Chain antibody [N1C3-3] used in Western Blot (WB). GTX112943
Anti-Ferritin Light Chain antibody [N1C3-3] used in IHC (Paraffin sections) (IHC-P). GTX112943
Anti-Ferritin Light Chain antibody [N1C3-3] used in Western Blot (WB). GTX112943

Cat. No. GTX112943

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Ferritin Light Chain products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rabbit, Bovine, Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ferritin Light Chain. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950317

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ferritin light chain , LFTD , NBIA3

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ferritin Light Chain antibody [N1C3-3] used in Western Blot (WB). GTX112943

GTX112943 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Light Chain antibody [N1C3-3] (GTX112943) diluted at 1:1000.

Anti-Ferritin Light Chain antibody [N1C3-3] used in IHC (Paraffin sections) (IHC-P). GTX112943

GTX112943 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using Ferritin Light Chain(GTX112943) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ferritin Light Chain antibody [N1C3-3] used in Western Blot (WB). GTX112943

GTX112943 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX112943 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169