Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Light Chain antibody [N1C3] (GTX101211) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using FTL(GTX101211) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Light Chain antibody [N1C3] (GTX101211) diluted at 1:1000.