GeneTex
United States (US)

Ferritin Light Chain antibody [N1C3]

GTX101211_39694_20160714_WB_M_liver_19011506_137.jpg
GTX101211_IHC_19011506_987.jpg
GTX101211_WB_19011506_616.jpg

Cat No. GTX101211

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rabbit, Bovine, Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.268mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ferritin Light Chain. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ferritin Light Chain,Lftd,Nbia3,Ftl

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101211_39694_20160714_WB_M_liver_19011506_137.jpg

GTX101211 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Light Chain antibody [N1C3] (GTX101211) diluted at 1:1000.

GTX101211_IHC_19011506_987.jpg

GTX101211 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using FTL(GTX101211) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101211_WB_19011506_616.jpg

GTX101211 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
12% SDS PAGE
GTX101211 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169