GeneTex
United States (US)

Ferritin antibody [521]

GTX15742_42_ELISA_19011519_817.jpg
GTX15742_41_ELISA_19011519_292.jpg
GTX15742_292_ICC-IF_19011519_902.jpg
GTX15742_43_ELISA_19011519_514.jpg

Cat No. GTX15742

Host Mouse
Clonality Monoclonal
Clone Name 521
Isotype IgG1
Application ICC/IF, IP, ELISA, RIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:50-1:200
IP Assay dependent
ELISA 1 μg/ml
RIA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.01M potassium phosphate, 2.5mM KCl, 0.135M NaCl, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Liver Ferritin

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LFTD,NBIA3

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
DATA IMAGES
GTX15742_42_ELISA_19011519_817.jpg

GTX15742 ELISA Image

Sandwich ELISA analysis of recombinant human ferritin light polypeptide (FTL) standards ranging from 1.6-1000ng/ml using GTX15742 Ferritin antibody [521] (detection antibody) at 1 µg/mL and Ferritin duck polyclonal antibody (capture antibody).

GTX15742_41_ELISA_19011519_292.jpg

GTX15742 ELISA Image

ELISA analysis of human Ferritin or BSA diluted in carbonate/bicarbonate buffer (1 µg/ml, 100µl/well) using GTX15742 Ferritin antibody [521] at 2 - 1000 µg/mL (serial diluted).

GTX15742_292_ICC-IF_19011519_902.jpg

GTX15742 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX15742 Ferritin antibody [521]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature

GTX15742_43_ELISA_19011519_514.jpg

GTX15742 ELISA Image

Sandwich ELISA analysis of recombinant human ferritin light polypeptide (FTL) standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted normal human plasma (B) using GTX15742 Ferritin antibody [521] (capture antibody) at 1 µg/mL and biotin-conjugated ferritin rabbit polyclonal antibody (detection antibody) at 1 µg/mL.

Package List Price ($)
$ 319