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Fetuin B antibody

Orthogonal
Anti-Fetuin B antibody used in IHC (Paraffin sections) (IHC-P). GTX112260
Anti-Fetuin B antibody used in Western Blot (WB). GTX112260
Anti-Fetuin B antibody used in Western Blot (WB). GTX112260
Anti-Fetuin B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112260
Anti-Fetuin B antibody used in Immunoprecipitation (IP). GTX112260
Anti-Fetuin B antibody used in Western Blot (WB). GTX112260
Anti-Fetuin B antibody used in Western Blot (WB). GTX112260
Anti-Fetuin B antibody used in Western Blot (WB). GTX112260
Anti-Fetuin B antibody used in Western Blot (WB). GTX112260

Cat. No. GTX112260

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fetuin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

mouse plasma , rat plasma , U87-MG , SK-N-SH , IMR32 , SK-N-AS

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fetuin B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172475

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fetuin B , 16G2 , Gugu , IRL685

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fetuin B antibody used in IHC (Paraffin sections) (IHC-P). GTX112260

GTX112260 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Fetuin B(GTX112260) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fetuin B antibody used in Western Blot (WB). GTX112260

GTX112260 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody (GTX112260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fetuin B antibody used in Western Blot (WB). GTX112260

GTX112260 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody (GTX112260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fetuin B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112260

GTX112260 ICC/IF Image

Fetuin B antibody detects Fetuin B protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N_SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fetuin B protein stained by Fetuin B antibody (GTX112260) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Fetuin B antibody used in Immunoprecipitation (IP). GTX112260

GTX112260 IP Image

Immunoprecipitation of Fetuin B protein from SK-N-SH whole cell extracts using 5 μg of Fetuin B antibody (GTX112260).
Western blot analysis was performed using Fetuin B antibody (GTX112260).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Fetuin B antibody used in Western Blot (WB). GTX112260

GTX112260 WB Image

Fetuin B antibody detects Fetuin B protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fetuin B antibody (GTX112260) diluted by 1:1000.

Anti-Fetuin B antibody used in Western Blot (WB). GTX112260

GTX112260 WB Image

Orthogonal

The data was published in the 2022 in Sci Rep. PMID: 35896788

Anti-Fetuin B antibody used in Western Blot (WB). GTX112260

GTX112260 WB Image

Orthogonal

The data was published in the 2022 in Sci Rep. PMID: 35896788

Anti-Fetuin B antibody used in Western Blot (WB). GTX112260

GTX112260 WB Image

Orthogonal

The data was published in the 2022 in Sci Rep. PMID: 35896788

REFERENCE

Application Reference

Wang D et al. Sci Rep 2022; 12 (1) : 12869 Hepatokine Fetuin B expression is regulated by leptin-STAT3 signalling and associated with leptin in obesity.
Peter A et al. Am J Physiol Endocrinol Metab 2017; : ajpendo.00262.2017 The Hepatokines Fetuin-A and Fetuin-B are up-regulated in the State of Hepatic Steatosis and may differently impact on Glucose Homeostasis in Humans.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169