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Fgf8a antibody

Anti-Fgf8a antibody used in IHC (Whole mount) (IHC-Wm). GTX128126
Anti-Fgf8a antibody used in IHC (Whole mount) (IHC-Wm). GTX128126
Anti-Fgf8a antibody used in IHC (Frozen sections) (IHC-Fr). GTX128126

Cat. No. GTX128126

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-Fr, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Fgf8a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Fgf8a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885712

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibroblast growth factor 8a , ace , cb110 , etID309886.13 , fgf-8 , fgf8 , id:ibd5031 , wu:fb73a06

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-Fgf8a antibody used in IHC (Whole mount) (IHC-Wm). GTX128126

GTX128126 IHC-Wm Image

fgf8a antibody detects fgf8a protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
fgf8a antibody (GTX128126) dilution: 1:200.
Anti-Fgf8a antibody used in IHC (Whole mount) (IHC-Wm). GTX128126

GTX128126 IHC-Wm Image

Fgf8a antibody detects Fgf8a protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Fgf8a stained by Fgf8a antibody (GTX128126) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-Fgf8a antibody used in IHC (Frozen sections) (IHC-Fr). GTX128126

GTX128126 IHC-Fr Image

The data was published in the journal Front Neurosci in 2019.PMID: 31680844

REFERENCE

Application Reference

Dash S et al. Development 2022; 149 (12) Nucleolin loss of function leads to aberrant Fibroblast Growth Factor signaling and craniofacial anomalies.
Giffen KP et al. Front Neurosci 2019; 13 : 1117 Expression of Protein-Coding Gene Orthologs in Zebrafish and Mouse Inner Ear Non-sensory Supporting Cells.
Application : IHC-Fr
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169