APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , H1299 , NIH-3T3 , Rat2
Specificity/Sensitivity
IP/MS validation was supported by references (PMID:30377378)
Predict Reactivity
Zebrafish, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
fibrillarin , FIB , FLRN , Nop1 , RNU3IP1
Cellular Localization
Nucleus , nucleolus
Background
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101807 WB Image
Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX101807) dilution: 1:1000
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GTX101807 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fibrillarin(GTX101807) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX101807 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
12% SDS PAGE
GTX101807 diluted at 1:1000
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GTX101807 IP Image
Fibrillarin antibody immunoprecipitates FBL protein in IP experiments.
IP samples: HeLa nuclear extract
A. 35 μg HeLa nuclear extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FBL protein by 4 μg Fibrillarin antibody (GTX101807)
10 % SDS-PAGE
The immunoprecipitated FBL protein was detected by Fibrillarin antibody (GTX101807) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX101807 WB Image
Sample (30 ug of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101807 diluted at 1:1000
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REFERENCE
REVIEW
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