GeneTex
United States (US)

Fibrillarin antibody

IP/MS
GTX101807_39645_WB_R_19011506_148.jpg
GTX101807_39645_IFA_19011506_542.jpg
GTX101807_WB_19011506_250.jpg
GTX101807_39645_IP_19011506_572.jpg
GTX101807_WB_M_19011506_929.jpg

Cat No. GTX101807

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
See all Fibrillarin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , H1299 , NIH-3T3 , Rat2

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377378)

Predict Reactivity

Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibrillarin,Fib,Flrn,Nop1,Rnu3Ip1,Fbl

Cellular Localization

Nucleus , nucleolus

Background

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101807_39645_WB_R_19011506_148.jpg

GTX101807 WB Image

Fibrillarin antibody detects FBL protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Fibrillarin antibody (GTX101807) dilution: 1:1000
GTX101807_39645_IFA_19011506_542.jpg

GTX101807 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fibrillarin(GTX101807) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX101807_WB_19011506_250.jpg

GTX101807 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
12% SDS PAGE
GTX101807 diluted at 1:1000
GTX101807_39645_IP_19011506_572.jpg

GTX101807 IP Image

Fibrillarin antibody immunoprecipitates FBL protein in IP experiments.
IP samples: HeLa nuclear extract
A. 35 μg HeLa nuclear extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FBL protein by 4 μg Fibrillarin antibody (GTX101807)
10 % SDS-PAGE
The immunoprecipitated FBL protein was detected by Fibrillarin antibody (GTX101807) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX101807_WB_M_19011506_929.jpg

GTX101807 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX101807 diluted at 1:1000

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169