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Fibrillarin antibody

Anti-Fibrillarin antibody used in Western Blot (WB). GTX32604
Anti-Fibrillarin antibody used in Immunoprecipitation (IP). GTX32604
Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32604

Cat No. GTX32604

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($329)
See all Fibrillarin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibrillarin , FIB , FLRN , Nop1 , RNU3IP1

Cellular Localization

Nucleolus

Background

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Fibrillarin antibody used in Western Blot (WB). GTX32604

GTX32604 WB Image

WB analysis of various samples using GTX32604 Fibrillarin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Fibrillarin antibody used in Immunoprecipitation (IP). GTX32604

GTX32604 IP Image

IP analysis of HeLa cell lysate using GTX32604 Fibrillarin antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32604

GTX32604 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32604 Fibrillarin antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 329