APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
fibrillarin , FIB , FLRN , Nop1 , RNU3IP1
Cellular Localization
Nucleolus
Background
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX32604 IP Image
IP analysis of HeLa cell lysate using GTX32604 Fibrillarin antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000
|
|
|
GTX32604 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX32604 Fibrillarin antibody.
Blue : DAPI
Dilution : 1:100
|
|
|
GTX32604 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX32604 Fibrillarin antibody.
Blue : DAPI
Dilution : 1:200
|
|
|
GTX32604 WB Image
WB analysis of various sample lysates using GTX32604 Fibrillarin antibody.
Dilution : 1:1000
Loading : 25μg per lane
|
REFERENCE
There are currently no references for Fibrillarin antibody (GTX32604). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Fibrillarin antibody (GTX32604). Be the first to share your experience with this product.
