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Fibrillarin antibody

Anti-Fibrillarin antibody used in Immunoprecipitation (IP). GTX65859
Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65859
Anti-Fibrillarin antibody used in IHC (Paraffin sections) (IHC-P). GTX65859
Anti-Fibrillarin antibody used in Western Blot (WB). GTX65859
Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65859
Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65859

Cat. No. GTX65859

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all Fibrillarin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 100-321 of human FBL (NP_001427.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibrillarin , FIB , FLRN , Nop1 , RNU3IP1

Cellular Localization

Nucleolus

Background

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Fibrillarin antibody used in Immunoprecipitation (IP). GTX65859

GTX65859 IP Image

IP analysis of HeLa cell lysate using GTX65859 Fibrillarin antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65859

GTX65859 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX65859 Fibrillarin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Fibrillarin antibody used in IHC (Paraffin sections) (IHC-P). GTX65859

GTX65859 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX65859 Fibrillarin antibody.
Dilution : 1:100

Anti-Fibrillarin antibody used in Western Blot (WB). GTX65859

GTX65859 WB Image

WB analysis of various sample lysates using GTX65859 Fibrillarin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65859

GTX65859 ICC/IF Image

ICC/IF analysis of C6 cells using GTX65859 Fibrillarin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Fibrillarin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65859

GTX65859 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX65859 Fibrillarin antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399