Fibrinogen antibody

Cat. No. GTX135332

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX135332 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Fibrinogen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909843

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibrinogen alpha chain , Fib2

Cellular Localization

Secreted

Background

This gene encodes the alpha subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, the encoded preproprotein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. [provided by RefSeq, Jan 2016]

Database

Gene ID: 2243 FGA
UniProt: P02671 FGA

Research Area

DATA IMAGES

GTX135332 ICC/IF Image

Fibrinogen antibody detects Fibrinogen protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen stained by Fibrinogen antibody (GTX135332) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

GTX135332 IHC-P Image

Fibrinogen antibody detects secreted Fibrinogen protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Fibrinogen stained by Fibrinogen antibody (GTX135332) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX135332 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Fibrinogen antibody (GTX135332) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty