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Fibrinogen antibody

Anti-Fibrinogen antibody used in IHC (Paraffin sections) (IHC-P). GTX26666
Anti-Fibrinogen antibody used in IHC (Paraffin sections) (IHC-P). GTX26666
Anti-Fibrinogen antibody used in Western Blot (WB). GTX26666
Anti-Fibrinogen antibody used in Western Blot (WB). GTX26666

Cat. No. GTX26666

Reference 3

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Fibrinogen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:10000
ICC/IF Assay dependent
IHC-P 1:500
ELISA 1:20000-1:100000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

90 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fibrinogen collected from human plasma

Conjugation

Unconjugated

RRID

AB_377990

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibrinogen alpha chain , Fib2

Cellular Localization

Secreted

Background

Fibrinogen exists as a soluble dimer (340 kDa) in plasma. Each monomer is composed of an alpha chain (63.5 kDa), a beta chain (56 kDa), and a gamma chain (47 kDa). Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body.

Database

Research Area

DATA IMAGES

Anti-Fibrinogen antibody used in IHC (Paraffin sections) (IHC-P). GTX26666

GTX26666 IHC-P Image

IHC-P of Goat Anti-Fibrinogen antibody (GTX26666). Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.

Anti-Fibrinogen antibody used in IHC (Paraffin sections) (IHC-P). GTX26666

GTX26666 IHC-P Image

IHC-P analysis of human liver tissue using GTX26666 Fibrinogen antibody.
Dilution : 1:500

Anti-Fibrinogen antibody used in Western Blot (WB). GTX26666

GTX26666 WB Image

WB analysis of reduced human fibrinogen using GTX26666 Fibrinogen antibody.
Loading : 1 μg
Dilution : 1:3000

Anti-Fibrinogen antibody used in Western Blot (WB). GTX26666

GTX26666 WB Image

Western Blot of Goat anti-Fibrinogen antibody (GTX26666). Lane 1: Fibrinogen under reducing conditions. Load: 1 μg per lane. Primary antibody: Fibrinogen antibody at 1:3,000 for overnight at 4ºC.

REFERENCE

Application Reference

Gutierrez-Pe?a M. et al. Clinical Kidney Journal 2021; 14 (4) : 1197–1206 High prevalence of end-stage renal disease of unknown origin in Aguascalientes Mexico: role of the registry of chronic kidney disease and renal biopsy in its approach and future directions.
Bill Cheng et al. RSC Adv 2018; 8 (28) : 15471-15479 The formulation and characterization of 3D printed grafts as vascular access for potential use in hemodialysis.
Application : ICC/IF
Reactivity : Human
Cheng B. et al. RSC Adv. 2018; 8 : 15471-15479 The formulation and characterization of 3D printed grafts as vascular access for potential use in hemodialysis.
Application : ICC/IF
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399