Fibrinogen beta antibody [C1C3]

Name: Fibrinogen beta antibody [C1C3]
Brand: GeneTex
SKU: GTX113694
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-Fibrinogen beta antibody [C1C3] used in Western Blot (WB). GTX113694

Anti-Fibrinogen beta antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113694

Anti-Fibrinogen beta antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX113694

Cat. No. GTX113694

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Fibrinogen beta products

View product citations for antibody GTX113694 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:50000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

mouse plasma , rat plasma

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrinogen beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036930

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibrinogen beta chain , HEL-S-78p

Cellular Localization

Secreted

Background

The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq]

Database

Gene ID: 2244 FGB
UniProt: P02675 FGB

Research Area

DATA IMAGES

GTX113694 WB Image

Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] (GTX113694) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113694 WB Image

Rat tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] (GTX113694) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113694 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Fibrinogen beta(GTX113694) antibody at 1:200 dilution.

GTX113694 IHC-P Image

Fibrinogen beta antibody [C1C3] detects Fibrinogen beta protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Fibrinogen beta antibody [C1C3] (GTX113694) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113694 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen beta antibody [C1C3] (GTX113694) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chuang YC et al. J Immunol 2011; 187 (12) : 6483-90 Dengue virus-induced autoantibodies bind to plasminogen and enhance its activation.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW