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Fibrinogen gamma antibody [HL1395]

Recombinant antibody
Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835
Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835
Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835
Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835
Anti-Fibrinogen gamma antibody [HL1395] used in IHC (Paraffin sections) (IHC-P). GTX636835

Cat. No. GTX636835

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1395

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Fibrinogen gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:100000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910083

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibrinogen gamma chain

Cellular Localization

Secreted

Background

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835

GTX636835 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835

GTX636835 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody [HL1395] used in Western Blot (WB). GTX636835

GTX636835 WB Image

Mouse plasma and rat plasma (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Fibrinogen gamma antibody [HL1395] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636835

GTX636835 ICC/IF Image

Fibrinogen gamma antibody [HL1395] detects Fibrinogen gamma protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fibrinogen gamma stained by Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:50.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Fibrinogen gamma antibody [HL1395] used in IHC (Paraffin sections) (IHC-P). GTX636835

GTX636835 IHC-P Image

Fibrinogen gamma antibody [HL1395] detects secreted Fibrinogen gamma protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Fibrinogen gamma stained by Fibrinogen gamma antibody [HL1395] (GTX636835) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169