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Fibrinogen gamma antibody

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX113436
Anti-Fibrinogen gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX113436
Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX113436
Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113436

Cat No. GTX113436

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human plasma , HepG2 sup. , Mouse plasma

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrinogen gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036931

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibrinogen gamma chain

Cellular Localization

Secreted

Background

The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX113436

GTX113436 WB Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein by Western blot analysis. Human plasma and HepG2 conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX113436) diluted at 1:2000.

Anti-Fibrinogen gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX113436

GTX113436 IHC-P Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Fibrinogen gamma antibody (GTX113436) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Fibrinogen gamma antibody used in Western Blot (WB). GTX113436

GTX113436 WB Image

Fibrinogen gamma antibody detects Fibrinogen gamma protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Fibrinogen gamma antibody (GTX113436) diluted at 1:1000.

Anti-Fibrinogen gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113436

GTX113436 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrinogen gamma (GTX113436) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169