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Fibroblast antibody [1B10]

Cat No. GTX11333

Host

Mouse

Clonality

Monoclonal

Clone Name

1B10

Isotype

IgM

Application

WB, IHC-Fr, FACS, Cytotoxic Assay

Reactivity

Human
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
Cytotoxic Assay 1:500
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

cultured human thymic fibroblasts

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. It is also useful for flow cytometry, immunohistochemistry and immunoblotting.

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379