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Fibroblasts/Epithelial antibody [D7-FIB]

Anti-Fibroblasts/Epithelial antibody [D7-FIB] used in Flow cytometry (FACS). GTX11208

Cat No. GTX11208

Host

Mouse

Clonality

Monoclonal

Clone Name

D7-FIB

Isotype

IgG2a

Application

IHC-Fr, FACS, IP

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-Fr 1/100
FACS 1/50-1/200
IP Assay dependent

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Recommend the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Product Note

Clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.This antibody is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human foreskin fibroblasts.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Fibroblasts/Epithelial antibody [D7-FIB] used in Flow cytometry (FACS). GTX11208

GTX11208 FACS Image

FACS analysis of KG1 cells using GTX11208 Fibroblasts/Epithelial antibody [D7-FIB].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379